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Chemicals
FAM disinfectant (Evans Vanodine International).
Citric acid (standard grade, any make).
Antibiotics
Eagle's Minimum Essential Medium (Eagle's MEM): This can be formulated according to standard tissue culture protocols or, alternatively, supplied complete as Minimum Essential Medium Eagle (HEPES Modification), from Sigma Ltd (500 ml).
Biological Reagents
Standard reference viruses: Stocks of standardised reference virus strains are grown in tissue culture and stored at -20% after addition of an equal volume of glycerol. The stocks are repeatedly titrated to obtain a running mean titre which is constantly updated each time an assay, including virus titration, is run.
Standard reference sera: Stocks of standardised bovine convalescent sera for each reference virus are maintained. The running mean titre of the reference serum is derived by repeated titration and is updated as described above.
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